Tags: Anti-herbivore defense, dendritic trichome, extrafloral nectary, flavonoids, glandular hair, glycerides, indirect defense, leaf development, morphology, optimal defense hypothesis, Paulownia tomentosa, shoot development, paulownia Panama
Sawa Kobayashi1,*, Teigo Asai2, Yoshinori Fujimoto2 and Shiro Kohshima1
1Department of Bioscience and Biotechnology
2 Department of Chemistry and Materials Science, Tokyo Institute of Technology, Meguro, Tokyo 152-8551, Japan
Background and Aims: Recent studies have shown that small structures on plant surfaces serve ecological functions such as resistance against herbivores. The morphology, distribution, chemical composition and changes during shoot and leaf development of such small structures were examined on Paulownia tomentosa.
Methods: The morphology and distribution of the structures were studied under light microscopy, and their chemical composition was analyzed using thin-layer chromatography and high-performance liquid chromatography. To further investigate the function of these structures, several simple field experiments and observations were also conducted.
Key Results: Three types of small structures on P. tomentosa were investigated: bowl-shaped organs, glandular hairs and dendritic trichomes. The bowl-shaped organs were densely aggregated on the leaves near flower buds and were determined to be extrafloral nectarines (EFNs) that secrete sugar and attract ants. Nectar production of these organs was increased by artificial damage to the leaves, suggesting an anti-herbivore function through symbiosis with ants. Glandular hairs were found on the surfaces of young and/or reproductive organs. Glandular hairs on leaves, stems and flowers secreted mucilage containing glycerides and trapped small insects. Secretions from glandular hairs on flowers and immature fruits contained flavonoids, which may provide protection against some herbivores. Yellow dendritic trichomes on the adaxial side of leaves also contained flavonoids identical to those secreted by the glandular hairs on fruits and flowers. Three special types of leaves, which differed from the standard leaves in shape, size and identity of small structures, developed near young shoot tips or young flower buds. The density of small structures on these leaf types was higher than on standard leaves, suggesting that these leaf types may be specialized to protect young leaves or reproductive organs. Changes in the small structures during leaf development suggested that leaves of P. tomentosa are primarily protected by glandular hairs and dendritic trichomes at young stages and by the EFNs at mature stages.
Conclusions: The results indicate that P. tomentosa protects young and/or reproductive organs from herbivores through the distribution and allocation of small structures, the nature of which depends on the developmental stage of leaves and shoots.
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